Nutritional reconstruction in an early modern population: Searching for a relationship between dental microwear and bone element composition.

BACKGROUND: Observing the microwear patterns of the dental crowns enamel surface can provide information on the ingredients and structure of the food consumed, but also on eating habits and lifestyle. Major role in reconstructing the diet and lifestyle of past populations is played by the analysis of alkaline earth metals, such as strontium, barium, zinc and calcium. Ba and Sr are indicators of the consumption of vegetables, plants (cereals and legumes) and marine organisms. This study aims to assess dietary diversity and identify its components based on microscopic techniques and chemical analyses of material from early modern archaeological sites in Wroclaw, Poland. METHODS: The material consisted of 36 permanent molars and the intrasternal parts of 122 first ribs, collected from 6 Wroclaw early modern cemeteries. Tooth microwear was evaluated on Scanning electron microscopy images, with Microwear 4.02 software. Bone chemical composition (Ca, P, Ba, Sr content) was evaluated with mass spectrometry. RESULTS: Most lines were present on the teeth from St. Mary Magdalene Cemetery, with the lowest average number of lines observed on the teeth from St. Barbara Cemetery. The Ca/P ratios calculated for different sites formed two clusters that allows to distinguish two groups of archaeological sites with different bone preservation status. Number of differences in Ba/Sr, Sr/Ca, Ba/Ca ratios was found between disctinct archaeological sites. A number of correlations were found between the concentration of the chemical elements, but no statistically significant correlation was found between the microwear characteristics and the proportion of the elements studied. Some strong correlations were found between microwear features and the Ca/P ratio. CONCLUSIONS: the different values of Sr/Ca, Ba/Ca and Ba/Sr ratios indicate different diets in the different communities. The data obtained indicate a mixed diet, with a relatively high proportion of animal products throughout Wroclaw. Conclusions should be treated with caution due to secondary diagenesis. The lack of interdependence between microwear and chemical composition characteristics suggests that the two methods should be considered complementary and not overlapping, as they provide different insights into the diets of past populations. The comparison of microwear between different sites should always take into account secondary diagenesis and the burial environment, as these influence the characteristics of microwear.

Therapeutic Potential of Dental Pulp Stem Cells According to Different Transplant Types.

Stem cells are unspecialised cells capable of perpetual self-renewal, proliferation and differentiation into more specialised daughter cells. They are present in many tissues and organs, including the stomatognathic system. Recently, the great interest of scientists in obtaining stem cells from human teeth is due to their easy availability and a non-invasive procedure of collecting the material. Three key components are required for tissue regeneration: stem cells, appropriate scaffold material and growth factors. Depending on the source of the new tissue or organ, there are several types of transplants. In this review, the following division into four transplant types is applied due to genetic differences between the donor and the recipient: xenotransplantation, allotransplantation, autotransplantation and isotransplantation (however, due to the lack of research, type was not included). In vivo studies have shown that Dental Pulp Stem Cells (DPSCs)can form a dentin-pulp complex, nerves, adipose, bone, cartilage, skin, blood vessels and myocardium, which gives hope for their use in various biomedical areas, such as immunotherapy and regenerative therapy. This review presents the current in vivo research and advances to provide new biological insights and therapeutic possibilities of using DPSCs.

Does image file transfer, exposure time and optimization algorithm affect digital intraoral radiographs?

BACKGROUND: The transfer of digital radiographic images is widely practiced. Digital image processing can influence the perception of image quality. The question arises as to how exposure, internal image adjusting algorithms and image file transfer affect the optical density of digital radiographs. OBJECTIVES: To evaluate the influence of exposure time, optimization and file transfer on digital radiographs. MATERIAL AND METHODS: Calibration patterns were formed and a series of digital radiograms were recorded under standardized conditions. The radiographs were exported and then imported into the same software. Three groups of radiographs were analyzed: A - images originally performed and recorded in the software; B - images imported after exporting an optimized image; and C - images imported after exporting an image without optimization but with measurements of the density of the marked regions of interest. RESULTS: An increase of the exposure time decreases optical density. The optimization algorithm increases contrast and decreases differences between exposure times. Long exposures affect the visibility of objects with low optical density. After importing the images in Group B, there was a risk of using the optimization algorithm twice. When optimization was not performed, there were no differences between Groups B and A. In Group C, there was no risk of doubling the optimization algorithm. CONCLUSIONS: The transfer of digital radiographs can exert an influence on the optical density values. To avoid the risk of image distortion, files should be exported without image optimization.

Diagnodent and VistaCam may be unsuitable for the evaluation of dental caries in archeological teeth.

OBJECTIVES: The aim of the study was to compare the usefulness of fluorescence-based caries detection systems (Diagnodent and VistaCam) for the assessment of carious lesions on archeological molars. MATERIALS AND METHODS: The study material consisted of teeth from the Cemetery of St. Mary Magdalene (Cmentarz sw. Marii Magdaleny) in Wroclaw, Poland. A sample of 178 permanent molars from 38 skulls were examined. Five surfaces of teeth (occlusal, mesial, distal, buccal, and lingual) were assessed on either basically cleaned or sandblasted teeth. Six diagnostic methods were used to detect carious lesions: the visual classification of the International Caries Detection and Assessment System (ICDAS II), fluorescent methods (Diagnodent and VistaCam), X-ray, cone beam computed tomography and histological sections. The sensitivity and specificity of the methods were determined using receiver operating characteristic (ROC) curves and the correlation between the severity of dental caries and the readouts obtained with each method. RESULTS: In most cases, Diagnodent and VistaCam yielded unsatisfactory specificity and sensitivity values. The area under curve (AUC) values in ROC curves for Diagnodent and Vistacam were lower than the AUC values obtained for the ICDAS II visual classification. CONCLUSIONS: According to our results, in the case of archeological teeth, neither Diagnodent nor VistaCam can be regarded as a better diagnostic method than the ICDAS II visual classification of caries.

Influence of physiological stress on the presence of hypoplasia and fluctuating asymmetry in a medieval population from the village of Sypniewo.

This study aims to estimate the levels of physiological stress in the medieval rural population of Sypniewo by evaluating patterns of fluctuating asymmetry (FA) and enamel hypoplasia (EH), and provide information on the influence of physiological stress during the prenatal and perinatal period on early childhood development. Stress is defined as any external or internal condition that challenges homeostasis of an organism. FA is associated with physiological stress occurring mainly during prenatal development and early childhood. The level of FA is thought to reflect the intensity of the stressor(s). EH is caused by physiological stress such as nutritional instability during the first years of life. The studied material consisted of 126 skulls from the village of Sypniewo (Poland). Cranial radiographs were taken in postero-anterior (P-A) and basal views. The images were scanned and calibrated. Measurements of the cranium were used to estimate FA. The presence of EH was assessed using standard anthropological methods The highest levels of FA were observed in the region of the cranial base. EH was observed in 29% of individuals from the rural skeletal series. There was no statistically significant correlation between FA and EH occurrence or between sex and the studied stress indicators.

[Caries of permanent dentition in medieval inhabitants of Wroclaw]. / Próchnica zebów stalych u sredniowiecznych mieszkanców Wroclawia.

INTRODUCTION: The study of dentition plays an important role in the reconstruction of the diet and in assessment of the overall health and living conditions of paleopopulations. The aim of this study was to determine the condition of permanent dentition of medieval inhabitants of Wroclaw basing on the prevalence and intensity of caries in permanent dentition. MATERIAL AND METHODS: The material consisted of 1156 permanent teeth from 118 skulls recovered from two medieval cemeteries in Wroclaw: the parish cemetery at the St. Elisabeth Church (13th-14th century) and the cemetery in Olbin (12th-13th century). Two age classes were formed taking into account anthropologic assessment and group size. The younger class consisted of material up to the age of 35 years; the remaining skulls were assigned to the older class. The prevalence and incidence of caries was determined. RESULTS: The prevalence and intensity of caries was 56.91% and 15.7%, respectively. Carious lesions predominated in males and in the older age class. CONCLUSIONS: The prevalence and intensity of caries in permanent dentition did not differ from other medieval populations and increased with age. High prevalence of caries reflects a high proportion of carbohydrates in the diet of medieval inhabitants of Wroclaw, their high socioeconomic status, and poor oral hygiene.